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News:

Starting NOW to the end of 2018, Sinica users get a 50% off discount on Biacore 8K measurements. Please submit samples through BCF reservation system.

To reflect the cost of maintenance, we adjusted Sinica instrument charges to NT$1000 and NT$2000 per day for Biacore T100 and T200 respectively, starting 2019.


Biacore Webinar:


Recent Upgrade:

Please refer to the T100 2.1.0 control software release notes for details.

BCF stock:

* Amine Coupling Kit
* 7mm tubes
* Desorb solutions

Available chips for testing nonspecific binding:

* C1 chip
* CM3 chip
* CM5 chip
* SA chip
* NTA chip

 

GE Biacore T200

Biacore T100 Biacore system is a series of Surface Plasmon Resornance (SPR) instruments. The instrument contains a flow system, a temperature control unit and an optical system to measure the molecular interactions occuring on sensor chips in real-time, with/without special labeling. This technique is capable for binding kinetics (on/off rate constants), affinity (equilibrium constants)and thermodynamic studies.

Important Notification:

(2018-10-26) The front door of Biacore T100 is broken and the position of injector needle is off. Service is called. Sorry for the inconvenience.

(2018-10-01) Starting NOW to the end of 2018, Sinica users get a 50% off discount on Biacore 8K measurements. Please submit samples through BCF reservation system.

(2018-10-05) To reflect the cost of maintenance, we adjusted Sinica instrument charges to NT$1000 and NT$2000 per day for Biacore T100 and T200 respectively, starting 2019.

(2018-05-14) We are replacing chip insertion unit of Biacore T200. Sorry for the inconvenience.

(2018-03-01) Free Biacore 8K sample tests available for BCF Biacore users (Sinica users only). Please submit your samples through BCF reservation system. Offer ends March 31st, 2018.

(2018-01-04) Chip insertion band of Biacore T200 is replaced.

(2017-11-22) Chip insertion module of Biacore T200 is recalibrated.

(2017-11-22) Biacore T200 is not available due to chip insertion and ejection error. Sorry for the inconvenience.

(2017-11-22) Biacore T200 is not available due to chip insertion and ejection error. Sorry for the inconvenience.

(2017-09-06) The temperature control units on both Baicore T200 and T100 are getting old and the cooling rate is not within instrument specification. Temperature below 25℃ is not recommended. Longer waiting time might be required for temperature to stabilize. Please check with Dr. Jao before planning your experiments.

* (2017-06-07) Biacore T200 is ready for reservation.

* (2017-05-26) Biacore T200 is not available due to noise baseline. Service is called.

* (2016-12-29) The Fc4 of Biacore T200 is clogged. The instrument is not available. Sorry for the inconvenience.

* (2016-12-09) The degasser of Biacore T200 is replaced. The instrument is ready for reservation.

* (2015-03-19) The control computer of Biacore T200 is replaced. The instrument is ready for reservation.

* (2015-03-03) The control computer of Biacore T200 is broken. Therefore, the instrument is not available. Sorry for the inconvenience.

* (2014-11-03) We are running out of caps for 7mm tubes. Please bring your own.

* (2014-02-17) The simplified start up and shutdown protocol has been modified.

* (2014-02-13) Biacore T100-IPMB is restored and ready for reservation. The chip insertion module has been replaced.

* (2014-01-28) Biacore T200 is restored and ready for reservation. However, Biacore T100-IPMB is shut down for its chip-insertion module.

* (2014-01-24) The chip of Biacore T200 was jammed inside. Service is called.

* (2013-05-23) Biacore T200 is ready for reservation.

* (2013-05-15) The autosampler of Biacore T200 was broken. Replacing injection unit service is scheduled to May 23rd. The instrument is expected to back on line on May 25th.

* (2013-01-02) Biacore T100 - IPMB is ready for reservation. Instrument usage rate is about half of using Biacore T200.

* (2012-12-07) Biacore T200 is ready for reservation. The leaking IFC was replaced.

* (2010-12-01) The new design of sealing plastic cover supplied by for 96-well plates is safe to use.

* Please use sealing foils with no glue applied inside of the wells. Otherwise, the needle can get clogged and will not deliver samples correctly.

title Selected Applications

Binding specificity
Binding kinetics
Active protein concentration
Molecular Recognition
Ligand fishing
Structure-function Relationship

title Specification

No. of flow cells
4
Flow rate
1 - 100 μl/min
Injection volume
2 - 350 μl
Required volume
injection volume + 20 - 50 μl
Flow cell volume
0.06 μl
Sample/reagent capacity
1x96/384-well plate+33 vials
Temperature range
10*-45 ℃ (*BCF instruments)
Refractive index range
1.33 - 1.39
Baseline noise
<0.03 RU (typical)
ka
1E3 - 3E9/Ms for proteins
1E3 - 5E7/Ms for small molecule
kd
1E-5 - 1 /s
Sample concentration
>10pM
Minimum molecular weight
<100Da

For more detail, please refer to Biacore T200 web page.

title Suggested Reading:

Biacore T100 Instrument Handbook.

Biacore T100 Software Handbook.

Rich RL and Myszka DG, 2008, Survey of the year 2007 commercial optical biosensor literature, J. Mol. Recognit., 21, 355-400.

Rich RL and Myszka DG, 2007, Survey of the year 2006 commercial optical biosensor literature, J. Mol. Recognit., 20, 300-366.

Schasfoort RBM and Tudos AJ (eds.), 2007, Handbook of Surface Plasmon Resonance. RSC Publishing.

Rich RL and Myszka DG, 2006, Survey of the year 2005 commercial optical biosensor literature, J. Mol. Recognit., 19, 478-534.

Single-Cycle Kinetics:
Karlsson R, Katsamba PS, Nordin H, Pol E and Myszka DG, 2006, Analyzing a kinetics titration series using affinity biosensors, Anal. Biochem., 349, 136-147.

Fragment Based Screening / Small Molecules:
Anthony M. Giannetti, 2011, From experimental design to validated hits, Methods in Enzymology, 493, 169-218.